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  1. Reference materials for microbiology and pathogens

    The regular occurrence of food borne pathogen related outbreaks in the EU, the ongoing struggle with pathogens in health care and public health as well as the security threat related to the potential misuse of pathogens emphasise the need for an adequate ...

    02 September 2015

  2. Possible genetic events producing colistin resistance gene mcr-1

    A commentary to the article Liu Y-Y, Wang T, Walsh TR, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study Lancet Infect Dis 2015; published online ...

    12 March 2016

  3. Characterization of Ricin and R. communis Agglutinin Reference Materials

    Ricinus communis intoxications have been known for centuries and were attributed to the toxic protein ricin. On the basis of its availability, toxicity, ease of preparation and the current lack of medical countermeasures ricin gained attention as potentia ...

    18 February 2016

  4. Genomic and Phenotypic Alterations of the Neuronal-Like Cells Derived from Human Embryonal Carcinoma Stem Cells (NT2) Caused by Exposure to Organophosphorus Compounds Paraoxon and Mipafox

    Organophosphorus compounds (OPs) are pesticides of worldwide use due to the acute insecticidal effects mediated by the inhibition of esterases of the central nervous system (mainly acetylcholinesterase and neuropathy target esterase (NTE)). OPs need to in ...

    16 January 2014

  5. An inter-laboratory validation of a multiplex dipstick assay for four classes of antibiotics in honey

    In this paper, we report the inter-laboratory validation (ILV) of a recently developed indirect competitive multiplex dipstick (Bee4sensor®) which is capable of the simultaneous detection of residues of some of the most frequently detected antibiotic resi ...

    26 September 2013

  6. Functional analysis of the integration host factor site of the sigma54 Pu promoter of Pseudomonas putida by in vivo UV imprinting

    The integration host factor (IHF) of Pseudomonas putida connects cell growth to transcriptional activity of distinct promoters. The IHF site of the sigma54 promoter Pu of the TOL (m-xylene biodegradation) plasmid pWW0 of P. putida has been examined to def ...

    29 May 2013

  7. Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress.

    High content omic techniques in combination with stable human in vitro cell culture systems have the potential to improve on current pre-clinical safety regimes by providing detailed mechanistic information of altered cellular processes. Here we investiga ...

    07 March 2013

  8. Analytical approaches for MCPD esters and glycidyl esters in food and biological samples: A review and future perspectives

    Esters of 2- and 3-monochloropropane-1,2-diol (MCPD) and glycidol esters are important contaminants of processed edible oils used as foods or food ingredients. This review describes the occurrence and analysis of MCPD esters and glycidol esters in vegetab ...

    25 January 2013

  9. Proficiency testing in analytical chemistry, microbiology and laboratory medicine: working discussions on current practice and future directions

    A summary of the working group (WG) discussions on proficiency testing (PT) and external quality assessment (EQA) held at the EURACHEM Workshop, Istanbul, 3–6 October 2011, is provided. The six WGs covered a range of issues concerned with current practice ...

    07 November 2012

  10. CCQM-K86/P113.1: Relative quantification of genomic DNA fragments from a biological tissue

    Key comparison CCQM-K86 was performed to demonstrate and document the capacity of interested national metrology institutes (NMIs) and designated institutes (DIs) in the determination of the relative quantity of two specific genomic DNA fragments present i ...

    20 July 2012

  11. Comparison of therapeutic efficacy and biodistribution of 213Bi- and 211At-labeled monoclonal antibody MX35 in an ovarian cancer model

    Introduction: The purpose of this study was to compare the therapeutic efficacy and biodistribution of the monoclonal antibody MX35 labeled with either 213Bi or 211At, both α-emitters, in an ovarian cancer model. Methods: One hundred female nude BALB/c (n ...

    17 April 2012

  12. What type of work are you involved in the cancer therapy research?

    The cancer research program at JRC-ITU focuses on all stages of developing targeted alpha therapy (TAT) from bench to bedside, including the development of innovative methods for the production of alpha emitters, pre-clinical studies in vitro and in vivo, ...

    30 April 2014

  13. Standardisation of Pluripotent Stem Cell Cultures for Toxicity Testing

    Introduction: Pluripotent stem cell (PSC) lines offer a unique opportunity to derive various human cell types which can be exploited for human safety assessments in vitro and as such contribute to modern mechanistically oriented toxicity testing. Areas co ...

    17 April 2012

  14. A mesenchymal stromal cell line resistant to paclitaxel that spontaneously differentiates into osteoblast-like cells

    The mesenchymal stromal cell line SR- 4987 has been established in our laboratory from the bone marrow of BDF/1 mice. Recent information on mesenchymal stem cells biology and the need to deal with well-characterized cell lines suggest to critically consid ...

    05 November 2011

  15. Molecular signatures induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells

    Using the Hey3Met2 human ovarian cancer cell line, we previously found the RNASET2 gene to possess a remarkable in vivo tumor suppressor activity, although no in vitro features such as inhibition of cell proliferation, clonogenic potential, impaired growt ...

    17 April 2012

  16. Autofluorescence microscopy: a non-destructive tool to monitor mitochondrial morphology and toxicity

    Visualization of NADH by fluorescence microscopy makes it possible to distinguish mitochondria inside living cells allowing structure analysis of these organelles in a noninvasive way. Mitochondrial morphology is determined by the occurrence of mitochondr ...

    04 November 2011