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Central Core DNA Sequence Information System (CCSIS)

The Central Core DNA Sequence Information System (CCSIS) is an implementation of a DNA sequence database integrated with bioinformatics tools to give support to the European Union Reference Laboratory for Genetically Modified Food & Feed (EU-RL GMFF) as foreseen by the Commission Regulation (EC) No 641/2004.

The CCSIS is composed of sequence data and about 180 supporting bioinformatics applications, including in-house developed tools like the JRC GMO-Matrix. Sequences are stored into the relational database and made available via web through a sequence retrieval system. The quality controlled data include sequences publicly available from research and patent databases, in-house produced sequences from Next Generation Sequencing (NGS) and the GMO sequence data submitted to the EU-RL GMFF by applicant companies as part of their legal obligations. As these are business confidential information, access to CCSIS is strictly controlled.

The DNA sequences are manually annotated (complemented with additional description) following international sequence standard formats of the International Nucleotide Sequence Database Collaboration.

The CCSIS runs on High Performance Computing servers, wholly or partly dedicated to Bioinformatics, thus allowing analyses to be carried out quickly like sequence similarity searches to assess the specificity of GMO detection method.

In parallel to the sequence data hosted in CCSIS, the JRC maintains the EU Database of Reference Methods for GMO Analysis, GMOMETHODS database. This database contains all information needed by laboratories for implementing the described GMO-detection methods. Only methods that are validated according to international standards are included.