Report on the Verification of the Performance of GHB614 and LLCotton25 Event-specific PCR-based Methods Applied to DNA Extracted from Stack Cotton GHB614 x LLCotton25

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Report on the Verification of the Performance of GHB614 and LLCotton25 Event-specific PCR-based Methods Applied to DNA Extracted from Stack Cotton GHB614 x LLCotton25
Abstract: 

An application was submitted by Bayer CropScience AG to request the authorisation of genetically modified cotton stack GHB614 x LLCotton25 (tolerant to glufosinate ammonium and glyphosate-containing herbicides) and all sub-combinations of the individual events as present in the segregating progeny, for food and feed uses, and import and processing, in accordance with articles 5 and 17 of Regulation (EC) N? 1829/2003 GM Food and GM Feed. The unique identifier assigned to GHB614 x LLCotton25 cotton is BCS-GH??2-5xACS-GH??1-3. The genetically modified cotton stack GHB614 x LLCotton25 has been obtained by conventional crossing of two genetically modified cotton events: GHB614 and LLCotton25, without any new genetic modification. The EU-RL GMFF has previously validated individually, and declared fit for purpose, the detection methods for the single events GHB614 and LLCotton25 (see http://gmo-crl.jrc.ec.europa.eu/StatusOfDossiers.aspx). In line with the approach defined by the ENGL (http://gmo-crl.jrc.ec.europa.eu/doc/Min_ Perf_Requirements_Analytical_methods.pdf) the EU-RL GMFF has carried out only an in-house verification of the performance of each validated method when applied to DNA extracted from GHB614 x LLCotton25. The herewith reported in-house verification study lead to the conclusion that the individual methods meet the ENGL performance criteria also when applied to DNA extracted from the GM cotton stack GHB614 x LLCotton25.

Authors
Authors: 
KREYSA Joachim, MAZZARA Marco, RISCHITOR Patricia Elena
Publication Year
Publication Year: 
2014
Type

Type:

Appears in Collections
Appears in Collections: 
Institute for Health and Consumer Protection
Science Areas
Keywords
JRC Institutes
Publisher
Publisher: 
Publications Office of the European Union
ISBN
ISBN: 
978-92-79-36786-1
ISSN
ISSN: 
1831-9424
DOI
URI
URI : 
Other Identifiers
Other Identifiers: 
EUR 26580
OPOCE LB-NA-26580-EN-N
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