Report on the Verification of the Performance of 1507, 59122, MON 810 and NK603 Event-specific PCR-based Methods applied to DNA extracted from Stack Maize 1507 x 59122 x MON 810 x NK603

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Report on the Verification of the Performance of 1507, 59122, MON 810 and NK603 Event-specific PCR-based Methods applied to DNA extracted from Stack Maize 1507 x 59122 x MON 810 x NK603
Abstract: 

An application was submitted by Pioneer Overseas Corporation to request the authorization of the genetically modified maize stack 1507 x 59122 x MON 810 x NK603, resistant against certain lepidopteran pests, protected against corn rootworm larvae, and glufosinate-ammonium and glyphosate tolerant, and all sub-combinations of the individual events as present in the segregating progeny, for food and feed uses, and import and processing, in accordance with articles 5 and 17 of Regulation (EC) N? 1829/2003 GM Food and GM Feed. The unique identifier assigned to 1507 x 59122 x MON 810 x NK603 maize is DAS-?15?7-1xDAS-59122-7xMON-??81?-6xMON-??6?3-6. The genetically modified maize line 1507 x 59122 x MON 810 x NK603 has been obtained by conventional crossing of four genetically modified single maize events: 1507, 59122, MON 810 and NK603 without any new genetic modification. The EU-RL GMFF has previously validated, and declared fit for purpose, the detection methods for the single events 1507, 59122, MON 810 and NK603 (see: http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm). In line with the approach defined by the ENGL (http://gmo-crl.jrc.ec.europa.eu/doc/Min_Perf_Requirements_Analytical_methods.pdf) the EU-RL GMFF therefore has carried out only an in-house verification of the performance of each validated method when applied to DNA extracted from 1507 x 59122 x MON 810 x NK603. The herewith reported in-house verification study lead to the conclusion that the individual methods meet the ENGL performance criteria also when applied to DNA extracted from the GM maize stack 1507 x 59122 x MON 810 x NK603.

Authors
Authors: 
JACCHIA Sara, KREYSA Joachim, MAZZARA Marco, SACCO Maria-Grazia
Publication Year
Publication Year: 
2013
Type

Type:

Appears in Collections
Appears in Collections: 
Institute for Health and Consumer Protection
Science Areas
JRC Institutes
Publisher
Publisher: 
Publications Office of the European Union
ISBN
ISBN: 
978-92-79-34674-3
ISSN
ISSN: 
1831-9424
DOI
URI
URI : 
Other Identifiers
Other Identifiers: 
EUR 26336
OPOCE LB-NA-26336-EN-N