The European Union Reference Laboratory for Feed additives, has been mandated by the Directorate General for Health and Consumers to organise a proficiency test (PT) among appointed National Reference Laboratories (NRLs). The aim of the PT was to assess the capacity of the NRLs to correctly determine 11 authorised coccidiostats in feed matrices at cross-contamination and additive levels. This report presents the results of the PT. Thirty-eight laboratories from 22 countries registered to this 2014 PT exercise. Laboratory results were rated using z- and ζ-scores (zeta-scores). Between 63 % and 79 % of the laboratories reported satisfactory results for monensin, narasin, lasalocid, diclazuril, decoquinate, halofuginone, robenidine, semduramicin and diclazuril FA. The laboratories also reported qualitative results as regards the presence of one or more of the other authorised coccidiostats. On the whole, the rate of false positive results was 3% for monensin and maduramicin and 0% for all the others.