Encouraged by the success of the first edition in 2012 the Joint Research Centre has re-published an entirely updated version of the EURL ECVAM Search Guide in 2013 (The Guide). The Guide has specifically been developed to support untrained database users in finding high quality information on relevant alternative strategies and methods to animal experiments on the web. The Guide provides examples of search procedures, suggested search terms and user guidance. It includes descriptions of relevant information resources and thesauri used for indexing publications as alternative approaches are concerned. A check list is moreover offered (the seven golden steps) to allow for searches in a structured and systematic way and to document them - a fundamental step in preparing a research project. Target users are scientists in biomedical sciences and toxicology, national authorities involved in animal protection and ethical committees verifying that all possible alternatives have been considered before conducting animal experiments.