Germination and Root Elongation Bioassays in Six Different Plant Species for Testing Ni Contamination in Soil

Abstract: 

Abstract In vitro short-term chronic phytotoxicity germination and root elongation test were applied to test the effects of nickel (Ni) in seed germination and root elongation in six plants species: Cucumis sativus (Cucurbitaceae), Lepidium sativum and Brassica nigra (Brassicaceae), Trifolium alexandrinum and Medicago sativa (Fabaceae), Phacelia tanacetifolia (Boraginaceae). A naturally Ni rich soil was used to compare the results obtained. Unlike root elongation, germinationwas not affected by Ni in any of the six species tested. EC50 values, calculated on the root elongation, showed that Ni toxicity decreases in the following order: P. tanacetifolia[B. nigra[C. sativus[ L. sativum[M. sativa[T. alexandrinum. The test conducted using soil elutriate revealed a significantly lower effect in both seed germination and root elongation when compared to the results obtained using untreated soil. Conversely, the test performed on soil confirmed the high sensitivity of C. sativus, P. tanacetifolia and L. sativum to Ni.

Authors
Authors: 
CONTI Federica, GARDI Ciro, VISOLI Giovanna, MENTA Cristina
Publication Year
Publication Year: 
2014
Type

Type:

Appears in Collections
Appears in Collections: 
Institute for Environment and Sustainability
Science Areas
Keywords
JRC Institutes
IES
Publisher
Publisher: 
SPRINGER
ISSN
ISSN: 
0007-4861
Citation
Citation: 
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY p. 490-496 no. 4 vol. 92
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