CCQM-K86/P113.1: Relative quantification of genomic DNA fragments from a biological tissue

Abstract: Key comparison CCQM-K86 was performed to demonstrate and document the capacity of interested national metrology institutes (NMIs) and designated institutes (DIs) in the determination of the relative quantity of two specific genomic DNA fragments present in a biological tissue. The study provides the support for the following measurement claim: "Quantification of the ratio of the number of copies of specified intact sequence fragments of a length in the range of 70 to 100 nucleotides in a single genomic DNA extract from ground maize seed materials". The study was carried out under the auspices of the Bioanalysis Working Group (BAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) and was piloted by the Institute for Reference Materials and Methods (IRMM) in Geel (Belgium). The following laboratories (in alphabetical order) participated in this key comparison: AIST (Japan), CENAM (Mexico), DMSc (Thailand), GLHK (Hong Kong), IRMM (European Union), KRISS (Republic of Korea), LGC (United Kingdom), MIRS/NIB (Slovenia), NIM (PR China), NIST (USA), NMIA (Australia), TÜBITAK UME (Turkey) and VNIIM (Russian Federation). The following laboratories (in alphabetical order) participated in a pilot study that was organized in parallel: LGC (United Kingdom), PKU (PR China), NFRI (Japan) and NIMT (Thailand). Good agreement was observed between the reported results of eleven participants.
Authors
Authors: 
CORBISIER Philippe, VINCENT Sandra, SCHIMMEL Heinz, KORTEKAAS Anna Maria, TRAPMANN Stefanie, BURNS M., BUSHELL C, AKGOZ M, AKYUREK S, DONG L., FU B, ZHANG L., WANG J., PEREZ URQUIZA M, BAUTISTA J.L., GARIBAY A, FULLER B, BAOUTINA Anna, PARTIS Lina, EMSLIE K., HOLDEN M., CHUM W.Y., KIM H.-H., PHUNBUA N, MILAVEC Mojca, ZEL Jana, VONSKY Maxim, KONOPELKO Leonid A., LAU T.L.T., YANG B, HUI M.H.K., YU A.C.H., VIROONUDOMPHOL D, PRAWETTONGSOPON C, WIANGNON K, TAKABATAKE R, KITTA K, KAWAHARASAKI M., PARKES H.
Publication Year
Publication Year: 
2011
Type

Type:

Science Areas
Publisher
Publisher: 
IOP PUBLISHING LTD
ISSN
ISSN: 
0026-1394
Citation
Citation: 
METROLOGIA p. [30 pages] no. 1A (Technical Supplement 2012) vol. 49