In the Guidance Document on Measurement Uncertainty for GMO Testing Laboratories two selected approaches for the estimation of measurement uncertainty are proposed. The first approach presented in detail is using data from collaborative trial in combination with in-house quality control data. In case no suitable collaborative trial data are available, an alternative approach, using data obtained on within-laboratory samples, is presented.
Are the GMO CRMs of IRMM checked for the presence/absence of other GM events than the one certified?
The GMO CRMs are gravimetric mixtures of pure GM and non-GM powders certified for a specific GM event as mass fraction (g/kg). IRMM verifies the purity of the two raw materials used for production with respect to the presence of the particular GM event that is certified. The CRMs are not certified for the absence or adventitious presence of other (contaminating) GM events.
The GMO CRMs can be used as positive controls for the certified GM event for instance to validate a screening method. However, the GMO CRMs should not be used as negative controls for any other GM event than the one certified. Also the blank material of each series is certified to contain less than the certified amount for a given GM event and the absence of this event can not be guaranteed.
For additional information IRMM has conducted a study to verify the GM composition of 33 raw materials used for the production of the GMO CRMs. Those raw materials have been tested by quantitative PCR for the presence/absence of 39 single GM targets known for 7 plant species. It needs to be noted that adventitious presence of other GM events is likely to be heterogeneously distributed over the batch of raw material and needs to be taken into account when comparing the results obtained so far. These results are summarised in
and provide an indication about possible adventitious presence.Institutes:
, reference material