FAQ

  1. Do you provide instructions how to perform PCR?
    30/04/2014

    Event-specific detection methods submitted under the provision of Art. 47 of Regulation EC 1829/2003 and their validation reports are accessible on the web site of the EURL for GM food and feed (EURL-GMFF) under the section "Status of dossiers". Detection methods used during the certification of a GMO reference material are listed in the certification report.

    Institutes: IRMM
    Keywords: GMO, reference material, measurement, quality, standard
  2. Which DNA extraction method should I use?
    30/04/2014

    Reliable results can only be guaranteed if an extraction method validated in a collaborative trial is used. Some extraction methods and their validation reports are accessible on the web site of the EURL for GM food and feed under the section "Status of dossiers".

    Institutes: IRMM
    Keywords: GMO, reference material, measurement, quality, standard
  3. Is the sequence information for GM varieties publicly available?
    30/04/2014

    The sequence information is usually patented by the producer and can in some cases be retrieved from the patent application. The sequence information itself is confidential and therefore not always accessible, whereas the primer and probe sequences targeted by the event-specific detection methods provided according to Art. 47 of Regulation EC 1829/2003 are accessible on the web site of the EURL for GM food and feed under the section "Methods database".

    Institutes: IRMM
    Keywords: GMO, reference material, measurement, quality, standard
  4. Can I purchase 100% GMO CRMs?
    30/04/2014

    IRMM only produces for selected GMO events a pure certified GMO reference material (nominal 100%). However, such materials may be available from other organisations or the intellectual property owner of the GMO event.

    Institutes: IRMM
    Keywords: GMO, reference material, measurement, quality, standard
  5. Can I use the GMO CRMs to calibrate my ELISA measurements?
    30/04/2014

    GMO reference materials from IRMM can be used as calibrants for DNA-based and protein-based methods. You should set-up a calibration with a reference material (calibrant) with known GM concentrations in order to be able to translate your test result into GM% m/m (mass fraction).

    Institutes: IRMM
    Keywords: GMO, reference material, measurement, quality, standard
  6. How to calculate the measurement uncertainty of PCR measurements?
    30/04/2014

    In the Guidance Document on Measurement Uncertainty for GMO Testing Laboratories two selected approaches for the estimation of measurement uncertainty are proposed. The first approach presented in detail is using data from collaborative trial in combination with in-house quality control data. In case no suitable collaborative trial data are available, an alternative approach, using data obtained on within-laboratory samples, is presented.

    Are the GMO CRMs of IRMM checked for the presence/absence of other GM events than the one certified?

    The GMO CRMs are gravimetric mixtures of pure GM and non-GM powders certified for a specific GM event as mass fraction (g/kg). IRMM verifies the purity of the two raw materials used for production with respect to the presence of the particular GM event that is certified. The CRMs are not certified for the absence or adventitious presence of other (contaminating) GM events.

    The GMO CRMs can be used as positive controls for the certified GM event for instance to validate a screening method. However, the GMO CRMs should not be used as negative controls for any other GM event than the one certified. Also the blank material of each series is certified to contain less than the certified amount for a given GM event and the absence of this event can not be guaranteed.

    For additional information IRMM has conducted a study to verify the GM composition of 33 raw materials used for the production of the GMO CRMs. Those raw materials have been tested by quantitative PCR for the presence/absence of 39 single GM targets known for 7 plant species. It needs to be noted that adventitious presence of other GM events is likely to be heterogeneously distributed over the batch of raw material and needs to be taken into account when comparing the results obtained so far. These results are summarised in this document file [70 KB] and provide an indication about possible adventitious presence.

    Institutes: IRMM
    Keywords: GMO, reference material, measurement, quality, standard