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Skin Sensitisation: the human Cell Line Activation Test (h-CLAT)

The human Cell Line Activation Test (h-CLAT) is used to contribute to the assessment of the skin sensitisation potential of chemicals.

The method addresses the third key event (KE3) of the skin sensitisation Adverse Outcome Pathway (AOP) by quantifying changes in the expression of cell surface markers associated with the process of activation of monocytes and dendritic cells.

The h-CLAT method was jointly developed by Shiseido and Kao Corporation in Japan and has been evaluated in a EURL ECVAM coordinated validation study and subsequently underwent independent peer review by the EURL ECVAM Scientific Advisory Committee (ESAC).

The method is adopted by the OECD as TG 442E.

The results of the validation study, the peer review of ESAC and our recommendations can be found on TSAR, the Tracking System for Alternative methods towards Regulatory acceptance.

Read more about the human Cell Line Activation Test (h-CLAT) on TSAR

Skin sensitisation

The human Cell Line Activation Test (h-CLAT)

Animal testing replacement

Validation study

Validation study outcomes

EURL ECVAM recommendations