EU FP6 Predictomics project: Short-term models assays for long-term toxicity (EU contract no 504761)
The aim of this European project was to develop a novel strategy for predicting chronic toxicity in the two most important target organs affected by drugs and xenobiotics, the liver and the kidney (Jennings et al., 2009). Advanced culture technology, genomic, proteomic and cytomic analysis were combined to detect the early events of cellular injury. The data generated were examined in view of the known pathophysiological changes linked to the chronic disease.
EURL ECVAM was part of the executive board and had an advisory role to ensure that methods were sufficiently well developed so that they could be considered for entering, eventually, future validation process.
EU FP6 Marie Curie Actions Research Training Network PULMO-NET: Pathogenesis of pulmonary disease (EU contract no MRTN-CT-2004-512229)
Pulmonet was created with the aim to improve methodological approaches to study lung function (such as fluid transport, secretion of surfactant, mucous or inflammatory mediators, and barrier/ transport functions) and dysfunction on a cellular and molecular level, gaining a better understanding of normal lung function, toxicity of environmental compounds, cell and tissue damage and generation of pulmonary disease.
The network consisted of experts from diverse fields, such as on lung proteins required for host defense and reduction of surface tension (surfactant proteins), lung cell isolation and culture, endotoxins and cell signalling, cytokines, inflammation and immune response, and use of cell lines (such as Calu-3) for barrier function and toxicity tests.
EURL ECVAM contributed to the network by setting up appropriate in vitro model(s) to study chemically induced damage to pulmonary barrier after exposure to substances of various kinds (Forti et al., 2010; Forti et al., 2011).
EU FP7 Predict-IV project: Profiling the toxicity of new drugs: a non animal-based approach integrating toxico-dynamics and biokinetics (EU Contract no. 202222)
The ultimate goal of Predict-IV was to develop a testing strategy integrating in vitro systems with knowledge of cell biology, mechanistic toxicology and in silico (pharmacokinetic) modelling.
EURL ECVAM lead activities on in vitro neurotoxicity testing, and contributed to OMICs profiling methods and on monitoring European efforts towards other non animal-based integrative approaches.
The relevant findings of this large scale integrating Project were published in a special issue of Toxicology in Vitro.
For more information, see Predict-IV website.
EU FP7 SEURAT-1: Towards the replacement of in vivo repeated dose systemic toxicity testing
This Research Initiative (2011-2016) was a first step to address the long term strategic target of "Safety Evaluation Ultimately Replacing Animal Testing (SEURAT)".
It was composed of six complementary research projects (SCR&Tox, HeMiBio DETECTIVE, COSMOS, NOTOX, ToxBank) and a coordination and support action (COACH) project.
The European Commission Joint Research Centre is a partner in SCR&Tox, DETECTIVE, COSMOS, and COACH.
For more information, see SEURAT-1 website.
H2020 EU-ToxRisk: An Integrated European 'Flagship' Programme Driving Mechanism-based Toxicity Testing and Risk Assessment for the 21st century
The EU-ToxRisk is designed as the follow up of the SEURAT 1 research initiative and will focus on two areas: repeated dose systemic toxicity, using the lung, kidney, liver and nervous system as examples of potential target organs; and developmental and reproductive toxicity.
For more information, see EU-Tox-Risk website.