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Eye Irritation: the Fluorescein Leakage (FL) test

This cell-based in vitro test method assesses the effects of test compounds on the integrity/permeability of epithelial cells and therefore may be useful as a measure of eye irritancy potential.

The method was validated in an EURL ECVAM retrospective validation study and is considered ready for use within a top-down approach in tiered eye irritation testing strategies.

In a wider testing strategy, this means it can be used to identify potential ocular corrosives and severe irritants from all other classes of water-soluble chemicals.

The results and full reports will be soon available on TSAR, the Tracking System for Alternative methods towards Regulatory acceptance.

Eye irritation

The Fluorescein Leakage (FL) test method

Animal testing replacement

Validation study

Validation study outcomes

EURL ECVAM recommendations