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Eye irritation: Bovine Corneal Opacity and Permeability (BCOP) assay

This assay directly assesses the effects of test chemicals on the opacity and permeability of bovine cornea.

It can be used as a measure for potential eye irritancy and as part of a wider testing strategy to classify chemicals and substances as ocular corrosives or severe irritants.

The method was assessed as part of a retrospective validation study that was conducted by ICCVAM-NICEATM in collaboration with EURL ECVAM.

Read more about BCOP method on ICCVAM-NICEATM website here.

Eye irritation

The Bovine Corneal Opacity and Permeability (BCOP) assay

Animal testing replacement

Validation study

Validation study outcomes

EURL ECVAM recommendations