EU Science Hub

Carcinogenicity: Three Cell Transformation Assays

These Cell Transformation Assays (CTAs) aim to predict the carcinogenic potential of chemicals.

The methods are designed to evaluate the effects of chemicals on the growth of specific cell types and their potential progression through a transformation process from being normal cells to fully malignant cells.

We coordinated a series of studies that aimed to standardise and subsequently evaluate three CTA protocols in terms of transferability and reproducibility.

Two methods focussed on the Syrian hamster embryo (SHE) cells (at pH 6.7 and pH 7.0) and one method used a mouse fibroblast cell line called BALB/c 3T3.

The results of the validation studies and full reports are availableon TSAR, the Tracking System for Alternative methods towards Regulatory acceptance.

Carcinogenicity

Three Cell Transformation Assays

Animal testing replacement

Validation study

Validation study outcomes

EURL ECVAM recommendations