Table 8-2: Local lymph node assay (LLNA) data on four fragrance substances and one essential oil
before and after air exposure, comparing the sensitisation potency of the pure (not oxidised)
substance with the potency of the oxidised.
| Substance |
CAS no. |
Doses % (w/v) vehicle: A:OO 4:1* |
EC3 value (% w/v) |
Reference |
Notes: Pure: Purified before testing as most commercially available fragrance substances are not
pure.
Not ox.: Not purified but used as it was delivered as this is a complex mixture and not a
specific substance.
Ox. x w: Oxidised by air exposure during x weeks.
* Acetone:olive oil. |
| D-Limonene (ox. 10 w) |
5989-27-5 |
1, 5, 25 |
3.0 |
(207) |
| D-Limonene (pure) |
5989-27-5 |
25, 50, 100 |
30 |
| Linalool (ox. 10 w) |
78-70-6 |
5, 10, 25 |
9.4 |
(127) |
| Linalool (ox. 45 w) |
78-70-6 |
2.5, 10, 25 |
4.8 |
| Linalool (pure) |
78-70-6 |
25, 50, 100 |
46.2 |
| Linalyl acetate (ox. 10 w) |
115-95-7 |
0.5, 10, 40 |
3.6 |
(128) |
| Linalyl acetate (pure) |
115-95-7 |
10, 30, 100 |
25 |
| Geraniol (ox. 10 w) |
106-24-1 |
1, 3, 6, 10, 20 |
4.4 |
(119) |
| Geraniol (ox. 45 w) |
106-24-1 |
0.5, 1, 3, 6, 10 |
5.8 |
| Geraniol (pure) |
106-24-1 |
5, 10, 15, 20, 30 |
22.4 |
| Lavender oil (ox. 10 w) |
|
1, 5, 10, 20, 50 |
11 |
(140) |
| Lavender oil (ox. 45 w) |
|
1, 5, 10, 20, 50 |
4.4 |
| Lavender oil (not ox.) |
|
5, 25, 100 |
36 |
Source:
SCCS,  Opinion on Fragrance allergens in cosmetic products, pages 61-62
|