| Mouse Swiss HIM/OG1 |
Host mediated assay with intraperitoneally inoculated Salmonella typhimurium strains TA1530, G46 |
dosing: 0.003, 0.3 or 3.0% H2O2 in milk for one week. 0.5 ml 0.3% H2 >O2 twice by gavage with a 2 h interval |
negative; H2O2 in milk positive; pure H2O2 |
a strong positive response for TA1530, a weak one for G46. |
Keck & Binder (1980) |
| Mouse inbread strain AB Jena Gat. |
cytogenetic assay with intraperitoneally inoculated tumour cells (S2 sarcoma, Ehrlich ascites, sarcoma 180) |
dosing: 1 ml of 0.01, 0.05, 0.1 or 0.5 M H2O2 i.p. 48 h after the implantation of the tumour cells. chromosomes were studied 48 h after the treatment. |
increased chromatid aberrations |
local effect; response presumed to depend on the presence or absence of RBCs. |
Schöneich (1967) |
| Rat Wistar, male |
In vivo - in vitro hepatocyte unscheduled DNA synthesis (UDS) |
H2O2 dosing: 0, 25 or 50 mg/kg by intravenous infusion of 0%, 0.1% or 0.2% water solution at a rate of 0.2 ml/min during approximately 30 min (=MTD) |
Negative |
exposure duration limited to 30 min. |
CEFIC (1997) |
| Mouse Swiss HIM/OF1 |
micronucleus assay of bone marrow polychromatic erythrocytes |
dosing: 0.003, 0.3 or 3.0% H2O2 in milk for 32 h (apparently also in water, % not given) |
Negative |
oral route, reporting unclear and incomplete. |
Keck & Binder (1980) |
| Mouse strain unkown |
micronucleus assay of bone marrow polychromatic erythrocytes |
single intraperitoneal injection of ½, 1/5, 1/25 or 1/100 LD50 dose of H2O2 |
Negative |
no experimental details given |
Liarskii et al. (1983) |
| Mouse C57BL/6NCr1BR |
micronucleus assay of bone marrow polychromatic erythrocytes |
H2O2 in drinking water at 0, 200, 1,000, 3,000 or 6,000 ppm for 2 weeks. Doses males: 0, 42.4, 164, 415 or 536 mg/kg |
negative, P/N ratio was not changed |
oral route |
Du Pont (1995) |
| |
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bw/day; females: 0, 48.5, 198, 485 or 774 mg/kg bw/day. |
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| Mouse Swiss OF1/ICO:OF1 (IOPS Caw) |
micronucleus assay of bone marrow polychromatic erythrocytes |
dosing: 0, 250, 500 or 1,000 mg/kg i.p. (25 ml/kg: 0, 1, 2 or 4% H2O2, respectively) Time of harvest 24 or 48 h |
negative, P/N was lower at 24 h, and at 48 h in the 250 and 1,000 mg/kg groups |
single i.p. injection |
CEFIC (1995) |
| Drosophila melanogasterr |
Drosophila SLRL test |
single dose of 3% H2O2 injected into male larvae |
Negative |
|
DiPaolo (1952) |
| Mouse SencarFemale |
Pre-screen forcarcinogenicity in target tissue (mouse skin) quantity of 8-OH-2’deoxyguanosine (DNA damage) mutations in codon 61 of cHa-ras gene epidermal hyperplasia and dermal cellularity changes |
Hydrogen peroxide 70% was applied to the skin of 10 female Sencar mice per dose group at dose levels of 10, 100, or 200 µmol in 200 µl of ethanol (i.e. 0.2-3.2% solutions) twice weekly for 4 weeks. Mice treated under the same conditions with DMBA (10 or 100 µmol/animal) or ethanol (200 µl) acted as positive and negative controls, respectively. The animals wer killed on days 2 or 4 after the last administration (5 mice on each day). The application sites were removed and after fixation and staining, epithelial and dermal thickness and dermal cellularity were determined visually by light microscopy. Non-phenol extraction of fresh frozen tissue was used to isolate DNA from animals killed 2 days after last dosing, and following digestion to nucleosides, 8-OH-2’deoxyguanosine (8-OH-dG) was quantified by HPLC. mutations in codon 61 of c-Ha-ras gene were determined using DNA isolated from paraffin blocks of whole skin. |
negative for all enpoints |
at the relatively low concentrations used hydrogen peroxide did not induce local in vivo genotoxicity and mutagenicity in the skin. |
Society for Plastic Industry (1997) |
