The Regions of Knowledge 2007-2013 (RoK) Programme was created to strengthen and develop research and innovation excellence in EU regions. Hence, the RoK focused on fostering regional growth and competitiveness, on enhancing regional investment in research and innovation, on facilitating transnational cooperation of clusters, and on supporting the emergence of European networks on the global stage. Furthermore, RoK tried to foster the inclusion of more regions into the ERA. This final evaluation of RoK, carried out by COWI, looks into the questions of the programme's relevance, efficiency, effectiveness, and the impact and sustainability in achieving this aim. It does this through a desk study, a questionnaire survey of RoK-project coordinators, and ten case studies of selected projects. The evaluation concludes that RoK has been a successful programme, although there are some risks regarding the sustainability of the RoK achievements.