Genetic implications in the production of rotifers in commercial finfish hatcheries
|| Universiteit Gent
Scientist responsible for the project
Prof. dr. PATRICK SORGELOOS
Belgium - BE
Phone: +32 (0)9 264 37 54
Fax: +32 (0)9 264 41 93
|Total EC contribution
||Rotifer clones will be isolated from culture samples and genetically characterised. Five clone mixtures will be used to evaluate the effect of culture conditions, methods and microbial flora on the genetic rotifer diversity. The use of resting eggs to maintain a high genetic diversity will be studied and the resting egg production and harvest will be optimised. The genetic selection in commercial cultures will be followed and the results will be presented to the industry in a workshop.
||WP1: The genetic characterisation of rotifer clones will enable the creation of mixtures of known rotifer clones needed in the other WPs.
WP2: The effect of culture methods on the genotypic diversity of cultured rotifers will document the differences of
the selection rate.
WP3: The effect of environmental conditions on the genotypic diversity of cultured rotifers will be determined in stable and variable biotic and abiotic parameters.
WP4: The effect of microbial stability on the genotypic diversity of the cultured rotifers will be studied in monoxenic and axenic cultures using a selected number of bacteria based on previous work.
WP5: The verification of abiotic and biotic conditions at pilot scale will be performed on different sites.
WP6: Sexual reproduction to provide resting eggs of selected clones will be optimised as these eggs can be used
as a cyst bank to increase the genotypic diversity in commercial hatcheries.
WP7: The follow-up of cultures in commercial hatcheries will provide ?field? experience and allow the scientific partners to observe the genetic drift in these cultures.
WP8: The obtained information will be assembled in a workshop for the industry and other scientists on the
effect of the rotifer culture method on the rotifer genetics and a possible way to prevent crashes.
|Progress to Date
||WP1: The collection of clones is established, protocols for preservation/sending/genetic identification have been set to point
Intercalibration between two partners for genetic analyses has been finalised.
WP2, 3, 4: These will be started in second year.
WP5: This is to be started in third year.
WP6: The preliminary tests have been done.
WP7: Samples have been taken every three days for a six month period at commercial hatchery, ready for genetic analyses
WP8: This is to be started in the third year.
||The first results are expected from month 12 onwards.