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  European Commission   > Research > Key Action 5
 
index by sub-area 5.1.1. Sustainable agriculture/Plant Systems
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Natural oxylipins and defence in ornamentals *

Contract nr: QLRT-CT-2000-02445
Project type: RS (Research and Technological Development Project)
Starting date:
Duration: 36 months
Total cost: 4,464,020 EUR
EC Contribution: 2,945,510 EUR
Scientific Officer:
Research topic: 5.1.1. Sustainable agriculture/Plant Systems

Abstract:
Aims: Engineer plant oxylipin synthesis in vitro to assess their usefulness as 'green' antimicrobial and pesticidal chemoprotectants. Manipulate plant oxylipin biosynthesis in vivo to modify defence reactions for improved resistance to pests and pathogens. Objectives: To produce an 'oxylipin tool kit' (recombinant enzymes and oxylipins in analytical quantities); to generate extensive knowledge on the in-vivo role of oxylipins in plant-pest and -pathogen interactions by generating a series of transgenic lines in Arabidopsis with altered oxylipin metabolism through overexpression and/or antisense suppression of the biosynthetic genes; to apply the gained knowledge and tools to protect ornamental plants. Outputs: Oxylipin tool kit; oxylipin profiles in wild type and transgenic Arabidopsis and Pelargonium plants; a collection of transgenic arabidopsis lines with altered oxylipin metabolism; and prototype transgenic lines of Pelargonium.

Objectives:
1) To produce a wide variety of oxylipins (oxygenated derivatives of polyunsaturated fatty acids) in large enough quantities for systematic evaluation of their chemoprotective properties.
2) To provide extensive information on alterations in oxylipin signatures of plants upon pest or pathogen challenge.
3) To generate a collection of transgenic Arabidopsis plants towards modification of oxylipin metabolism.
4) To analyse the consequences of manipulating oxylipin metabolic enzymes on oxylipin fluxes and profiles, plant gene expression and development, and resistance to pests and pathogens.
5) To test the efficacy of oxylipins as environmentally friendly chemoprotectants in greenhouse conditions.
6) To investigate the toxicology of selected oxylipins, and to formulate these compounds for application to soil-grown plants; to evaluate costs and marketability of candidate oxylipins.
7) To produce transgenic varieties from commercial Pelargonium with altered oxylipin metabolism.


Coordinator
Mariano ESTEBAN RODRIGUEZ
Centro nacional de biotecnologia
Madrid
SPAIN
Tel.: +34 915854503
Fax: +34 915854506
E-mail: mesteban@cnb.uam.es


Partners
  Mats HAMBERG
Karolinska Institutet, Medical biochemistry and biophysics
Stockholm
SWEDEN
Tel.: +46 87287640
Fax: +46 87360439
E-mail: mats.hamberg@mbb.ki.se
  Jose Alberto TORRES
Fundacion Promiva, Research and development
Boadilla del Monte
SPAIN
Tel.: +34 916166428
Fax: +34 916162605
E-mail: promiva@adenle.es
  Raymond BASTIDE
Université Paul Sabatier
Castanet-Tolosan
FRANCE
Tel.: +33 5 61556611
Fax: +33 5 61556470
E-mail: secdrre@adm.ups-tlse.fr
  Patrick MARTIN
Horticulture Research International, Dept of genetics and biotechnology, horticulture
Warwick
UNITED KINGDOM
Tel.: +44 1789470382
Fax: +44 1789470552
E-mail: patrick.martin@hri.ac.uk
  Dierk SCHEEL
Institute of Plant Biochemistry
Halle / Saale
GERMANY
Tel.: +49 3455582257
Fax: +49 3455582104
E-mail: dscheel@ipb-halle.de
  Mariano ESTEBAN RODRIGUEZ
Centro nacional de biotecnologia
Madrid
SPAIN
Tel.: +34 915854503
Fax: +34 915854506
E-mail: mesteban@cnb.uam.es
  Mary ANDERSON
John Innes Centre, Department of applied genetics
Norwich
UNITED KINGDOM
Tel.: +44 1603450244
Fax: +44 1603450016
E-mail: mary.anderson@bbsrc.ac.uk
  Marius J.A. DE BIE
Department of bio-organic chemistry, Bijvoet center for biomolecular research
Utrecht
THE NETHERLANDS
Tel.: +31 302533801
Fax: +31 302540980
E-mail: debie@nmr.chem.uu.nl
  Ulrich WOBUS
Institute of plant genetics and crop plant research, department of moleculare cell biology
Gatersleben
GERMANY
Tel.:
E-mail: wobusu@ipk-gatersleben.de