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Abstract:
The
proposal involves mapping QTL affecting milk production and associated
traits by selective DNA pooling, in Simmental (dual-purpose);
two Holstein and one (multicountry) Brown Swiss (dairy) cattle
populations; and in a unique Simmental population produced by
crossing a single F1 male (Red Holstein x Simmental) to Simmental
as recurrent parent. High-resolution mapping will be carried out
for some of the identified QTL, using selective recombinant genotyping
within, and identical-by-descent mapping across the various populations.
Allele frequency of QTL alleles, and marker haplotypes associated
with positive and negative QTL alleles of active sires will be
determined. Theoretical studies needed for use of QTL information
for marker-assisted selection (MAS) will be carried out. The end-product
will be a body of QTL map information and sire marker/QTL information,
and of validated methodologies for MAS for milk production traits
in dairy and dual-purpose cattle.
Objectives:
1.
To map QTL for rnilk yield and protein % in Holstein and Brown
Swiss (dairy), Simmental (dual-purpose), and Red Holstein x Simmental
advanced backcross.
2. To determine associated effects of the mapped QTL on protein
yield, fat yield, % fat, milk somatic cell count, days open, calving
difficulties and perinatal mortality.
3. To achieve high-resolution QTL mapping by selective recombinant
genotyping; and
4. by identical-by-descent mapping.
5. To develop and test methods to estimate frequency of QTL allele
in the population.
6. To determine marker-QTL phase in active élite sires of the
five populations.
7. To trace marker-phased QTL to progeny.
8. To develop a theoretical framework for incorporating marker-QTL
map information in a realistic marker assisted selection (MAS)
programme.
9. To develop a theoretical framework for evaluating the results
of a MAS programme, and apply this to the five resource populations
using the parameters obtained in this study.
Coordinator
Detlef VON FUCHS
Ludwig-Maximilians-Universitaet München
München
GERMANY
Tel.:
+49 8921803659
Fax:
+49 8921802985
E-mail:
detlef.fuchs@verwaltung.uni-muenchen.de
Partners
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Eran VARDI
The Hebrew University of Jerusalem
Jerusalem
ISRAEL
Tel.: +972 26586676
Fax: +972 25664740
E-mail: ruth@vms.huji.ac.il |
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Leopold MAERZ
Institut für Nutztierwissenschaften
Vienna
AUSTRIA
Tel.: +43 1476541000
Fax: +43 1476541019
E-mail: rektorat@mail.boku.ac.at |
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Giancarlo PRATELLA
University of Bologna, Dipartimento di protezione e valorizzazione agroalimentare, sez
Reggio Emilia
ITALY
Tel.: +39 0522290522
Fax: +39 0522290523
E-mail: vincenzo.russo@stpa.unibo.it |
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Alessandro BAGNATO
Università Degli Studi Di Milano, Istituto di Zootecnica
Milano
ITALY
Tel.: +39 022369441
Fax: +39 0270602227
E-mail: alessandro.bagnato@unimi.it |
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