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  European Commission   > Research > Key Action 5
 
index by sub-area 5.1.1. Sustainable agriculture/Plant Systems
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Feasibility of an EU plant health directive (77/93/EEC) diagnostic chip *

Contract nr: QLRT-CT-2000-02270
Project type: RS (Research and Technological Development Project)
Starting date:
Duration: 36 months
Total cost: 1,452,408 EUR
EC Contribution: 980,783 EUR
Scientific Officer:
Research topic: 5.1.1. Sustainable agriculture/Plant Systems

Abstract:
This project will investigate the feasibility of producing a diagnostic chip for the simultaneous detection of all the plant pests/pathogens present in the EU Plant Health Directive (77/93/EEC), using quarantine potato pests as a model system. The project will establish methods for detection of quarantine pathogens in plant tissue using gene chip technology. Up to 30 000 DNA probes (sequences from each of the organisms to be tested in one assay) can be arrayed on to a microscope slide - the 'gene chip'. The chip can be exposed to fluorescently labelled nucleic acid from the sample and fed into a micro-array reader to reveal if any of the pathogens are present. The project covers the whole process from preparing target genes, generating gene chips, preparing samples, hybridisation of samples, analysis of results and finally through to quarantine diagnosticians evaluating the technology.

Objectives:
The present project proposal aims to establish methodologies for the direct detection of quarantine pathogens of potato in suspect plant samples using gene chip technology. The 'gene chip' consists of a glass microscope slide with the gene sequences from each of the organisms that need to be detected in a single assay arrayed on its surface. Methods will be established for sample extraction, sample labelling, hybridisation and data capture. The two most important characteristics of a diagnostic protocol will be compared with traditional methods, i.e. sensitivity and specificity. The end-point of the project is a 'ring test' where, following a training course to facilitate technology transfer, the technology developed will be evaluated by quarantine diagnosticians.


Coordinator
Stephen HILL
Plant Health Group, Central Science Laboratory
York
UNITED KINGDOM
Tel.: +44 1904462227
Fax: +44 1904462250
E-mail: s.hill@csl.gov.uk


Partners
  Keith GOODEY
Centre for novel agricultural products, Department of biology, University of York
York
UNITED KINGDOM
Tel.: +44 01904432458
Fax: +44 01904432124
E-mail: kg8@york.ac.uk
  Dominique OTTOMANI
Unité Santé végétale et environnement
Antibes
FRANCE
Tel.: +33 4 93678801
Fax: +33 4 93678825
E-mail: ottomani@antibes.inra.fr
  Florentino JUSTE
Dep. proteccion vegetal y biotecnologia
Moncada (Valencia)
SPAIN
Tel.: +34 961391000
Fax: +34 961394453
E-mail: fjuste@ivia.es
  Erko STACKEBRANDT
DSMZ Plant Virus Division
Braunschweig
GERMANY
Tel.: +49 5312616352
Fax: +49 5312616418
E-mail: erko@dsmz.de
  Markus FRIESER
MWG-Biotech AG
Ebersberg
GERMANY
Tel.: +49 809282890
Fax: +49 809221084
E-mail: info@mwgdna.com