IMPORTANT LEGAL NOTICE - The information on this site is subject to a disclaimer and a copyright notice
Banner Research
English
 
  European Commission   > Research > Key Action 5
 
index by sub-area 5.1.2. Sustainable fisheries and aquaculture
index by country France
 

A functional genomic approach to measuring stress in fish aquaculture *

Contract nr: QLRT-CT-2000-02211
Project type: RS (Research and Technological Development Project)
Starting date:
Duration: 36 months
Total cost: 2,981,358 EUR
EC Contribution: 1,914,190 EUR
Scientific Officer:
Research topic: 5.1.2. Sustainable fisheries and aquaculture

Abstract:
Stress associated with fish aquaculture leads to a reduction performance and an increase in disease outbreaks. Improving the tolerance of fish to stressful procedures would have significant economic, environmental and welfare benefits.This project proposes a functional genomics approach to stock improvement. It will employ DNA microarray technology in tandem with whole animal physiology to identify genes whose expression is linked with stress resistance in rainbow trout. Expression profiles in selected tissues will be carried out in fish exposed to stress factors and will be used to isolate stress-responsive genes. This approach will be applied to characterise selected trout families (i) for high- and low-stress response to acute stress, and (ii) for freshwater growth performance.

Objectives:
The primary objective of this study is to develop whole animal gene expression profiles to identify candidate genes which are asociated with resistance to various stress factors in rainbow trout. Information obtained from genomic expression profiles of stressed fish is a necessary base for further development of a marker-assisted selection strategy. The primary goal will be achieved by utilising DNA microarray technology together with whole animal physiology to address several subsidiary objectives:
1. The gene expression profiles of selected tissues will be determined in fish exposed to standardised stress factors.
2. Genes whose expression is linked to adaptation response to stress will be identified.
3. The difference in gene expression profiles between selected trout, which display a divergent trait (stress responsiveness, growth, salinity adaptation) will be used to identify potential candidate markers of genotypic variation in stress responsiveness.


Coordinator
Bernard COQUET
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
Rennes
FRANCE
Tel.: +33 2 223485301
Fax: +33 2 22485300
E-mail:


Partners
  Jan EKMAN
UPPSALA UNIVERSITY
Uppsala
SWEDEN
Tel.: +46 184712627
Fax: +46 184716484
E-mail: Jan.Ekman@ebc.uu.se
  James FOX
THE UNIVERSITY OF LIVERPOOL
Liverpool
UNITED KINGDOM
Tel.: +44 1517942529
Fax: +44 1517942086
E-mail: J.F.Fox@liv.ac.uk
  Jane HARRISON
THE NATURAL ENVIRONMENT RESEARCH COUNCIL
Ambleside (Cumbria)
UNITED KINGDOM
Tel.: +44 01539442468
Fax: +44 01539446914
E-mail: jman@ceh.ac.uk
  Fred STEVENSON-ROBB
UNIVERSITY COURT OF THE UNIVERSITY OF ABERDEEN
Aberdeen
UNITED KINGDOM
Tel.: +44 1224272121
Fax: +44 1224272319
E-mail: f.stevenson-robb@abdn.ac.uk
  Joseph WATSON
NATIONAL UNIVERSITY OF IRELAND, GALWAY, IRELAND
Galway
IRELAND
Tel.: +353 91750343
Fax: +353 91526388
E-mail: joe.watson@nuigalway.ie