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Tools for the genetic improvement of sea bass. Construction and preliminary application of a medium density linkage and synteny map *

Contract nr: QLRT-CT-2000-01701
Project type: RS (Research and Technological Development Project)
Starting date:
Duration: 36 months
Total cost: 2,091,750 EUR
EC Contribution: 1,167,820 EUR
Scientific Officer:
Research topic: 5.1.2. Sustainable fisheries and aquaculture

Abstract:
Sea bass is the target of a growing aquaculture industry on the Mediterranean and the SEAtlantic coast. A conspicuous deficit in this industry is the absence of selection programmes and the appropriate scientific genetic base. For the first time, we will make a genetic and synteny map of sea bass which will provide the basis for an enhanced selection programme.

The proposal involves:
1) the breeding of a test panel of sea bass parents and their progeny;
2) the preparation of a genome bank, and the development of many markers of three different kinds;
3) the screening of the panel with these newly developed markers;
4) the preparation of a genotype data bank and the construction of the map proper; and
5) the demonstration of our project in an industrial environment. This objective information will directly benefit the aquaculture industry and, in the long run, fisheries management.


Objectives:
BASSMAP will prepare a medium density linkage and synteny map to improve the knowledge on inheritance in sea bass.

The aims to reach this goal are:
1) The breeding of haploid (mitotic), meiotic gynogenetic and F1 crosses of reference families.
2) To provide DNA from the reference families to other mapping projects in an attempt to speed up the acquisition of additional markers and genes on the map.
3) The development of a partially characterised Bacterial Artificial Chromosome library for the long-term support of mapping and comparative genomic in this species.
4) The development and anchoring of a genetic linkage map using a mixed strategy, including dominant (Amplified Fragment Length Polymorphisms) and co-dominant DNA microsatellite markers.
5) Development of a synteny map on the basis of Expressed Sequence Tags for comparative mapping.
6) A pilot study in an industrial environment to attract the attention of end-users in the field of quantitative trait loci.


Coordinator
André OSTERLINCK
KATHOLIEKE UNIVERSITEIT LEUVEN
Leuven
BELGIUM
Tel.: +32 16 324066
Fax: +32 16 324198
E-mail: gerard.cielen@doc.kuleuven.ac.be


Partners
  Aristidis BELLES
NIREUS CHIOS AQUACULTURE S.A.
Hiliadou
GREECE
Tel.: +30 16624280
Fax: +30 16626803
E-mail: chairman@nireus.com
  Christina BESTA
ARISTOTLE UNIVERSITY OF THESSALONIKI
Thessaloniki
GREECE
Tel.: +30 31996745
Fax: +30 31200392
E-mail: research@rc.auth.gr
  Sandro RABITTI
CONSIGLIO NAZIONALE DELLE RICERCHE
Venezia
ITALY
Tel.: +39 0412404711
Fax: +39 0415204126
E-mail: biomar@ibm.ve.cnr.it
  Carol ROSIE
ROSLIN INSTITUTE (EDINBURGH)
Roslin Midlothian
UNITED KINGDOM
Tel.: +44 1315274200
Fax: +44 1314400434
E-mail: carol.rosie@bbsrc.ac.uk
  Jen SHEARER
UNIVERSITY OF STIRLING
Stirling
UNITED KINGDOM
Tel.: +44 1786466692
Fax: +44 1786466680
E-mail: jms5@stir.ac.uk
  Eleftherios ZOUROS
INSTITUTE OF MARINE BIOLOGY OF CRETE
Iraklio
GREECE
Tel.: +30 81242022
Fax: +30 81241882
E-mail: zouros@imbc.gr