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  European Commission   > Research > Key Action 5
 
index by sub-area 5.1.1. Sustainable agriculture/Plant Systems
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Making plants resistant to plant parasitic nematodes: no access - no feeding

Contract nr: QLK5-CT-1999-01501
Project type: RS (Research and Technological Development Project)
Starting date: 01-02-2000
Duration: 48 months
Total cost: 3,940,481 EUR
EC Contribution: 2,978,656 EUR
Scientific Officer: Massimo BURIONI
Research topic: 5.1.1. Sustainable agriculture/Plant Systems

Abstract:
Building on preceding EU projects (BI04-CT96-0318, FAIRl-CT95-0905), potato and tomato plants will be generated with robust and durable resistances against root knot and cyst nematodes. The resistances will be based on the creation of multiple barriers. Because basic mechanisms of plant-nematode interactions will be studied and disrupted, the outcome of this project will also provide a means to engineer resistance against other plant parasitic nematodes. To increase the chance of public acceptance, the engineered resistance will be highly specific for the target organisms and the expression of the anti-nematode genes will be reduced to a minimum outside the sites of interest.

Objectives:
The objective of this proposal is to identify crucial elements in the interaction between plant parasitic nematodes and plants, and to exploit these elements for engineering broad and durable resistance against root knot and cyst nematodes by creating multiple barriers. First of all, the nematode will be prevented from migrating to the site where it starts the initiation of a feeding cell. Secondly - if it does this spot - it will be prevented from forminf a feeding cell. Proper targeting will be guaranteed because either nematode responsive elements from available promoters will be used or sets of promoters with common activity at the site of interest only. Finally, these promoters will be used to drive a very localised expression of multiple nematode-disturbing genes, and the resulting plants will be tested for cyst and root knot nematode resistance.


Coordinator
Hans HELDER
Wageningen University - Laboratory of nematology
Wageningen
THE NETHERLANDS
Tel.: +31 317 483136
Fax: +31 317 484254
E-mail: Hans.Helder@nema.dpw.wau.nl


Partners
  Rudy DEKEYSER
Flanders Interuniversity Institute for Biotechnology VZW
Gent
BELGIUM
Tel.: +32 9 244 66 11
Fax: +32 9 244 66 10
E-mail: rudy.dekeyser@vib.be
  Dominique OTTOMANI
Institut National de la Recherche Agronomique
Antibes
FRANCE
Tel.: +33 4 93678801
Fax: +33 4 93678825
  Roger KILLICK
The Scottish Crop Research Institute
Dundee
UNITED KINGDOM
Tel.: +44 1382 562731
Fax: +44 1382 562426
E-mail: apack@scri.sari.ac.uk
  Miguel OLMEDA FERNANDEZ
Universidad de Castilla - La Mancha
Toledo
SPAIN
Tel.: +34 967599259
Fax: +34 967599222
E-mail: molmeda@vin-ab.uclm.es
  Karin-Barbara SPINDLER
Christian-Albrechts-Universitaet zu Kiel
Kiel
GERMANY
Tel.: +49 431 880 3050
Fax: +49 431 880 1560
E-mail: dez132@zentr-verw.uni-kiel.de
  Wlodzimierz KLUCINSKI
Szkola Glowna Gospodarstwa Wiejskiego
Warsaw
POLAND
Tel.: +48 22 8438588
Fax: +48 22 8438588
E-mail: rektoraalfa.sggw@waw.pl
  Herman VAN MELLAERT
CropDesign N.V.
Zwijnaarde
BELGIUM
Tel.: +32 9 241 50 83
Fax: +32 9 241 50 89
E-mail: herman.vanmellaert@cropdesign.com
  Keith LINDSEY
University of Durham
Durham
UNITED KINGDOM
Tel.: +44 1913 747059
Fax: +44 1913 742417
E-mail: keith.lindsey@durham.ac.uk