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  European Commission   > Research > Key Action 5
 
index by sub-area 5.3.1. Multifunctional management of forests
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Enzyme discovery in hybrid aspen for fibre engineering

Contract nr: QLK5-CT-2000-00443
Project type: RS (Research and Technological Development Project)
Starting date: 01-01-2001
Duration: 48 months
Total cost: 3,809,265 EUR
EC Contribution: 2,593,161 EUR
Scientific Officer: Alexandros ARABATZIS
Research topic: 5.3.1. Multifunctional management of forests

Abstract:
The main aim is enzyme discovery from 8000 hybrid aspen EST-sequences for their eventual use in fibre engineering. The stepwise selection scheme includes:

1) Identification of genes highly expressed during wood formation using the microarray technology for rnRNA expression profiling,
2) Novel bioinformatics software for sensitive analysis of distant evolutionary relationships and modular structures of selected enzymes,
3) Heterologous expression and enzymatic characterization of selected enzymes,
4) Fibre analysis of Arabidopsis mutants in orthologous genes by reverse genetics,
5) Protein expression, localization and fibre analyses in transgenic aspen with up/doweregulated expression of selected genes,
6) Micropulping of selected fibre samples to correlate the enzymatic activities with final product properties.



Coordinator
Tuula TEERI
Royal Institute of Technology, Department of Biotechnology
Stockholm
SWEDEN
Tel.: +46 8 790 6103
Fax: +46 8 224 601
E-mail: tuula@biochem.kth.se


Partners
  Maureen MacCann
John Innes Centre
Norwich
UNITED KINGDOM
Tel.: +44 1603 450000
Fax: +44 1603 501771
E-mail: maureen.maccann@bbsrc.ac.uk
  Björn SUNDBERG
Dept. of Forest Genetics and Plant Physiology
Umea
SWEDEN
Tel.: +46 90 7866303
Fax: +46 90 7865901
E-mail: Bjorn.Sundberg@genfys.slu.se
  Mikael LINDSTRÖM
Swedish Pulp and Paper Research Institute
Stockholm
SWEDEN
Tel.: +46 8 6767281
Fax: +46 8 4115518
E-mail: mikael.lindstrom@stfi.se
  Antoni PLANAS
Institut Quimic de Sarria, Department of Organic Chemistry
Barcelona
SPAIN
Tel.: +34 932038900
Fax: +34 932056266
E-mail: aplan@iqs.es
  Malcom BENNETT
School of Biological Sciences of Nottingham
Nottingham
UNITED KINGDOM
Tel.: +44 115 951 3255
Fax: +44 115 951 3298
E-mail: Malcolm.Bennett@nottingham.ac.uk
  Wout BOERJAN
University of Gent, Department of Plant Genetics
Gent
BELGIUM
Tel.: +32 9 2645202
Fax: +32 9 2645349
E-mail: woboe@gengenp.rug.ac.be
  Hugues DRIGUEZ
CNRS-CERMAV
Grenoble
FRANCE
Tel.: +33 4 7603 7664
Fax: +33 4 7654 7203
E-mail: hugues.driguez@cermav.cnrs.fr
  Bernard HENRISSAT
AFMB-CNRS
Marseille
FRANCE
Tel.: +33 4 91 164516
Fax: +33 4 91 164536
E-mail: bernie@afmb.cnrs-mrs.fr