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Characterisation of Listeria monocytogenes to provide tools
to predict biofilm formation during cheese making

Contract number : QLK1-2002-02219
Contract type : Shared Cost Project
Total cost : Under negotiation
EC contribution : Under negotiation
Starting date : not yet determined
Duration : 36 Months
Scientific Officer : Dyanne Bennink
Project website : not yet available
Prof. Dr Peter Andrew
University of Leicester
Department of Microbiology and Immunology
University Road
LE1 9HN Leicester
United Kingdom
Tel.: +44-116-2522951
Fax: +44-116-2525030
E-mail: pwa @

The project is being undertaken in response to the need to control the presence of Listeria monocytogenes in the cheese making dairy. The persistence of L. monocytogenes in this environment will be correlated with its capacity for adaptation to acid and salt and its ability to form biofilm and resist disinfection. Identification of proteins involved in these physiological responses will allow identification of the corresponding gene. The data will be used to formulate advice on control of L. monocytogenes in the dairy. It will also provide tools that will predict the presence of strains with a high capacity to form biofilm in the dairy and hence are likely to become endemic. Finally it will provide evidence for which strain characteristics might be open to blockade as a strategy to prevent or remove biofilm.


The overall objectives are

  • To identify characteristics of Listeria monocytogenes that enable it to persist in cheese and within cheese-making dairies;
  • To elaborate a manual of advice and good practice for control L. monocytogenes in the dairies;
  • To provide evidence for which listerial proteins might be neutralised to prevent biofilm;
  • To provide tools for predicting which strains of L. monocytogenes are likely to colonise the dairies.


Instituto Nacional de Engenharia e Tecnologia Industrial
Departamento de Tecnologia das Industrias Alimentares
Estrada do Paço do Lumiar
Edificio S-N 22
1649-038 Lisboa
Loughborough University
Department of Chemical Engineering
Ashby Road
LE11 3TU Loughborough
United Kingdom
Universidade de Lisboa
Unit of Applied Biochemistry
Instituto de Ciencia Aplicada e Tecnologia da Faculdade de Ciencias
Campo Grande
1749-016 Lisbon
Universidade do Algarve
Faculdade de Engenharia de Recursos Naturais
Campus de Gambelas
8 000-117 Faro

Fifth Framework Programme

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