This proposal addresses isssues of quality management, traceability and safety during the production and processing of milk. It is proposed to design and develop novel sensing devices for a range of key analytes as indicators of milk contamination and milk processing efficiency. Faecal contamination and aflatoxin M1 detection will be monitored during fresh-milk collection. Then marker compounds as lactulose, lactose, lactosilated proteins and alkaline phosphatase and lactoperoxidase enzymes will be analyzed and quantified in real time using screen printed or composite biosensors and immunosensors coupled with flow-through or fia technology.
The objectives of this proposal are the development novel sensing devices for a range of key analytes, selected as indictor of milk contamination or milk processing efficiency. Faecal contamination and mycotoxin contamination will be measured with screen printed electrodes. Also the detection of marker enzymes as alkaline phosphatase and lactoperoxidase mid compounds lactose and lactulose, lactosilated proteins in real time, on-line, with effective cost instrumentation and directly 'in loco' will be the major objective of this proposal to assess quality, safety and traceability of freshly collected and processed milk.
- Faecal contamination sensor based on electrochemical detection using screen printed electrode technology;
- Immunosensor for aflatoxin M1;
- Integration of both the sensors into a robotic milking machine;
- Screen printed electrochemical sensors for AP and LPOX;
- Screen printed and/or biocomposite probes for lactose and lactulose;
- Immunosensor for lactosilated protein;
- Development of instrumentation ad hoc;
- Validation in operational milk processing plant.