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Robust chemical sensors and biosensors for rapid on-line identification
of freshly collected and processed milk

Contract number : QLK1-2001-01617
Contract type : Shared Cost Project
Total cost : € 1.305.425
EC contribution : € 879.528
Starting date : 1/12/2001
Duration : 36 Months
Scientific Officer : Dyanne Bennink
Project website : not yet available
Prof. Dr Giuseppe Palleschi
Università degli Studi di Roma "Tor Vergata"
Dipartimento di Scienze d Tecnologie Chimiche
Via della Ricerca Scientifica, 18
00133 Rome
Tel.: +39 06 72594423
Fax: +39 06 72594328
E-mail: giuseppe.palleschi @

This proposal addresses isssues of quality management, traceability and safety during the production and processing of milk. It is proposed to design and develop novel sensing devices for a range of key analytes as indicators of milk contamination and milk processing efficiency. Faecal contamination and aflatoxin M1 detection will be monitored during fresh-milk collection. Then marker compounds as lactulose, lactose, lactosilated proteins and alkaline phosphatase and lactoperoxidase enzymes will be analyzed and quantified in real time using screen printed or composite biosensors and immunosensors coupled with flow-through or fia technology.


The objectives of this proposal are the development novel sensing devices for a range of key analytes, selected as indictor of milk contamination or milk processing efficiency. Faecal contamination and mycotoxin contamination will be measured with screen printed electrodes. Also the detection of marker enzymes as alkaline phosphatase and lactoperoxidase mid compounds lactose and lactulose, lactosilated proteins in real time, on-line, with effective cost instrumentation and directly 'in loco' will be the major objective of this proposal to assess quality, safety and traceability of freshly collected and processed milk.

(expected) Results and achievements
  • Faecal contamination sensor based on electrochemical detection using screen printed electrode technology;
  • Immunosensor for aflatoxin M1;
  • Integration of both the sensors into a robotic milking machine;
  • Screen printed electrochemical sensors for AP and LPOX;
  • Screen printed and/or biocomposite probes for lactose and lactulose;
  • Immunosensor for lactosilated protein;
  • Development of instrumentation ad hoc;
  • Validation in operational milk processing plant.


Ente Nazionale per le Nuove Tecnologie, l'Energia e l'Ambiente
Biotechnology and Agriculture
Via Anguillarese 301
00060 S.Maria di Galeria - Rome
Università del Molise
Dipartimento di Scienze e Tecnologie Agro-Alimentari Ambientali e Microbiologiche
Via de Sanctis, Snc
86100 Campobasso
University of The West of England
Bristol Faculty of Applied Sciences
Frenchay Campus, Coldharbour Lane
BS16 1QY Bristol
United Kingdom
DeLaval International AB
PO Box 39
14721 Tumba
Universitat Autónoma de Barcelona
Química Analítica
Campus Universitari de Bellaterra
Bellaterra Cerdanyola del Vallés
Queen Mary University of London
Mile End Road
E1 4NS London
United Kingdom
S.P.A. Parmalat
Research Center
Via S. Vitale, 22
43038 Sala Baganza, Parma
Silsoe Research Institute
Wrest Park, Silsoe
MK45 4HS Bedford
United Kingdom

Fifth Framework Programme

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