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Traceability of DNA fragments throughout the food chain
by DNA/PNA technologies: Application to novel foods
DNA-TRACK

Contract number : QLK1-2000-01658
Contract type : Shared Cost Project
Total cost : € 2.928.110
EC contribution : € 1.787.510
Starting date : 01/01/2001
Duration : 36 Months
Scientific Officer : Rosanna d'Amario
Project website :
http://www.dsa.unipr.it/~foodhealth/
Coordinator
Dr Nelson Marmiroli
University of Parma
Parco Area delle Scienze
43100 Parma
Italy
Tel.: +39-0521 905606
Fax: +39-0521-905665
E-mail: marmirol @ ipruniv.cce.unipr.it
Background

The project concerns DNA detection in raw materials and foods. Traceability of DNA tracts through the food chain will be studied. The project involves well-established methods based on PCR, advanced PCR methods, and Real Time PCR, which allow precise quantification, and development of new method development of DNA microarrays. A new PNA-technology, actually aimed at biomedical diagnostics will be established as complementary to PCR, aimed at improving sensitivity, selectivity and increased detection limits in food. Validation of different methods will be performed, in order to provide updated criteria of choice in food control through the food chain. Application to genetically modified organisms (GMOs) will be performed. Food industries, retailer's groups, consumers' associations and universities are involved.

Objectives
  • to develop advanced analytical methods for DNA fragments targeting throughout the food chain;
  • to validate these methods at the level of a food network and to evaluate their applicability;
  • to establish a "de minimis" threshold of detection for these methods which can help in discriminating adventitious contamination;
  • to provide the technical innovation that will be utilised by decision makers and regulators to establishing the so called "negative list" i.e. the list related to products which have been excluded, because they should not contain DNA of alien origin;
  • All the above mentioned methods will be validated by partners who have a long experience in food sampling and food analysis.
(expected) Results and achievements
  • to develop advanced analytical methods for targeting DNA fragments throughout the food chain;
  • to validate these methods at the level of food network and to evaluate their applicability;
  • to establish a de minimis threshold of detection for these methods which can help in discriminating adventitious contamination;
  • to provide the technical innovation that will be utilised by decision makers and regulators to establishing the so called "negative list" i.e. the list related to products which have been excluded, because they should not contain DNA of alien origin;
  • to validate all the above mentioned methods by partners who have a long experience in food sampling and food analysis.

Partners

National Institute of Agricultural Botany
Huntingdon Road,
Cambridge CB3 0LE
United Kingdom
University of Applied Sciences
School of Engineering Valais
Route du Rawyl 47
1950 Sion
Switzerland.
Istituto di Tecnologie Biomediche
Consiglio Nazionale delle Ricerche
Via Fratelli Cervi 93
20090 Segrate Milano
Italy
PROGEO Soc. Coop. ARL
Via Asseverati 1
42029 Villa Masone (Reggio Emilia)
Italy
Max-Planck-Institut für Polymerforschung
Ackermannweg 10
55128 Mainz
Germany
Plantechno S.R.L
Via Staffolo 60
26040 Vicomoscano Cremona
Italy
University of Copenhagen
Department of Medical Biochemistry and Genetics
Blegdamsvej 3c
2200 Copenhagen N
Denmark
University of Parma
Department of Chemistry
Parco Area delle Scienze 17/A
43100 Parma
Italy


Fifth Framework Programme

PDF Version

:

Volume 1 (PDF 2.9 MB)

   

Volume 2 (PDF 1.9 MB)

 

Last update

:

23-09-2003



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