So far it is not possible to quantify reliably the constituents of fish or other seafood products. The methods used today rely on the quantification of substances highly susceptible to processing conditions. Due to its stability the quantification of DNA is a very promising attempt to overcome this problem. Practicable, fast and easy to use methods for quantification are in the interest of consumers and industry to prevent fraud and mislabelling. The technical progress in DNA-quantification offers the opportunity to develop methods applicable in routine analysis. The excellent access to the European market, to fisheries along with long experience in DNA analytic and state of the art equipment will provide the fundament to achieve protocols and procedures for high throughput identification of fish and seafood and a sensitive, reliable technique to quantify species in fish and seafood products.
- Improvement of DNA-based fish and seafood identification
- Development of reliable as well as sensitive quantification techniques for fish and seafood products. In recent years the growing interest in quantitative applications for the PCR has favoured the development of a large number of assay procedures suitable for this purpose. We will focus on two methodological approaches for quantitative PCR based on the use of fluorogenic probes. The remarkable technical and analytical progress made can now be exploited to gain routine analytical procedures.
- Evaluation of the developed techniques, protocols and procedures for identification and quantification by collaborative studies of the participants.
- Evaluation of the developed techniques in routine practice by end users.
- Collection of authentic organisms, relevant fish and other seafood products
- DNA-identification and quantification methods will be adopted to fish and seafood products
- DNA-database will be complemented with information relevant for fish and seafood products
- Primers and probes for fish and seafood identification; DNA-arrays-PCR-quantification exploiting the properties of competitive-, on-line-PCR and HPLC
- Ring trials among participants and end users for harmonisation and maximum practicability