IMPORTANT LEGAL NOTICE - The information on this site is subject to adisclaimerand acopyright notice
 
Contact   |   Search  
Graphical element Graphical element Graphical element Graphical element Graphical element Graphical element Graphical element Graphical element Graphical element
Cell factory - BAck to the homepage Graphic element Graphical element
Foreword Graphic element Graphical element
Introduction Graphical element
Index by Index by Area Index by Partners Index by Keywords Gr&phic element Graphical element
Volume 1 Graphical element
     
Graphical element Graphical element

PROduction of high value-added carotenoids and proVITamin A in cell factory crops (ProVitA)

   
Project

QLK3-2000-00809

Cell factory area

3.3.2

EU Contribution

1 869 285 Euro

Duration

36 months

Type

Research project

Starting date

01-03-2001

Keywords
carotenoids
cell factory crops
potato
tomato
Graphical element

Graphical element
ABSTRACT
Graphical element

Carotenoids are terpenoid pigments very widespread in nature. Dietary carotenoids are able to prevent chronic diseases. In a previous EU-financed effort, the current consortium has obtained genetically modified, high-beta-carotene, "golden" rice and tomatoes. The present project aims to extend this approach to high value-added carotenoids and crops of European interest. Isolation of new genes is envisaged, as well as agronomic, genomic and nutritional studies to address the "substantial equivalence" issue between GM and non-GM plants with altered carotenoid composition.

Graphical element
OBJECTIVES
Graphical element
  • To isolate plant structural and regulatory genes for the synthesis of antioxidant carotenoids.
  • To transform the tomato fruit into a cell factory for carotenoids: overproduction of lycopene, zeaxanthin, astaxanthin and lutein.
  • To transform the potato tuber into a cell factory for carotenoids: overproduction of lycopene and beta-carotene.
  • To compare, using DNA microarrays, genetically modified crops and classical mutants with altered carotenoid composition.
  • To determine the carotenoid bioavailability in GM crops.
  • To evaluate the agronomic performance of GM crops.

Graphical element
DESCRIPTION OF THE WORK
Graphical element

The workplan is organized into three workpackages:

WP1 Gene hunting: This deals with the isolation of the still-missing structural genes in carotenoid metabolism (epsilon-hydroxylase; aldehyde oxidase and glycosylase; tangerine; neoxanthin synthase). Regulatory genes, like tomato white flower and Arabidopsis genes affecting pigmentation and carotenoid gene promoter expression, will be also sought.

WP2 Genetic engineering and metabolic genomics: This deals with the modification of carotenoid content in tomato and potato, using genetically modified and mutant lines, and with the study, by microarray technology, of the genome expression profiles in such modified lines.

WP3 Nutritional and agronomic evaluation: This deals with the evaluation of the antioxidant and provitamin A activity of carotenoid-fortified plants using animal and in vitro models and with evaluation of the agronomic performance of genetically modified and mutant plants existing in the network and arising from workpackage 2.

Graphical element
DELIVERABLES
Graphical element
  • Novel plant genes for the synthesis of high value-added carotenoids.
  • Synthesis of novel carotenoids in tomato and potato.
  • Data on the genomic expression profile in GM plants.
  • Data on the bioavailability of carotenoids from GM plants.
  • Data on the agronomic performance of GM plants.

Graphical element
CONSORTIUM
Graphical element
COORDINATOR
  Prof. Giovanni Giuliano
Ente per le Nuove tecnologie
l'Energia e l'Ambiente (ENEA)
Casaccia Research Center
PO Box 2400
00100AD Roma, Italy
Tel: +39 630483192
Fax: +39 630483215
giulianog@casaccia.enea.it

PARTNERS
  Prof. Peter Bramley
Dept. of Biochemistry Royal Holloway
Univ. of London
TW20 OEX Egham, Surrey, United Kingdom
Tel: +44 1784443555
Fax: +44 1784434326
P.Bramley@rhbnc.ac.uk

Dr. Peter Beyer
Institut Biologie II
Univ. Freiburg
Schaenzlestraße 1
79104 Freiburg, Germany
Tel: +49 7612032529
Fax: +49 7612032675
beyer@uni-freiburg.de

Prof. Bilal Camara
Institut de Biologie Moleculaire
des Plantes CNRS
12, Rue du General Zimmer
67084 Strasbourg Cedex, France
Tel: +33 388417231
Fax: +33 388614442
Bilal.camara@ibmp-ulp.u-strasbg.fr

Prof. Hans Biesalski
Dept. Biological Chemistry & Nutrition
Univ. Hohenheim
Fruwirth 12
70593 Stuttgart, Germany
Tel: +49 7114594112
Fax: +49 7114593822
biesal@uni-hohenheim.de

Prof. Joseph Hirschberg
Dept. Genetics
The Hebrew University of Jerusalem
Givat Ram Campus
91904 Jerusalem, Israel
Tel: +972 26585662
Fax: +972 25633066
hirschu@vms.huji.ac.il

Dr. Marcel Kuntz
Université Joseph Fourier
PO Box 53
38041 Grenoble Cedex 9, France
Tel: +33 476514492
Fax: +33 476514336
marcel.kuntz@ujf-grenoble.fr

Dr. Rachel Drake
Zeneca Agrochemicals,
Jealot's Hill Research Station
RG42 6ET Bracknell, United Kingdom
Tel: +44 1344414201
Fax: +44 1344413886
rachel.drake@aguk.zeneca.com

Mr. Renato Faraone Mennella
FarMen SnC
V. Nazionale 360
80059 Torre del Greco (NA), Italy
Tel: +39 0818471662
Fax: +39 0818472466
info@farmen.com
Graphical element