Navigation path

CanTrain


Host-pathogen interaction systems as tools to identify antifungal targets in C. albicans and C. dubliniensis
EC contribution
: € 2.689.991
Duration
: 48 months
Starting date
: 01/03/2005
Funding scheme
: Marie-Curie Research Training Network
Keywords
: fungal infections, Candida sp., target screening, nutrient sensing, host-pathogen, morphogenesis, proteome analysis
Contract/Grant agreement number
:MRTN-CT-2004-512481
Project web-site
:-

Background:

One of the major tasks the Life Science industry faces in the coming years is to validate a plethora of targets for treatment of various diseases and the identification of novel lead compounds to generate novel pharmaceutics specifically acting on the targets identified. The "CanTrain" network will train both early and experienced researchers in the methodologies necessary for drug development, including target identification, target validation, development of cell-based screening assays all of which is required for drug screening and lead compound identification. For this purpose "CanTrain" addresses infectious diseases caused by fungal pathogens, especially strategies for the identification of novel antifungal drugs. Fungal infections have increased largely in the recent years, mostly due to immunocompromised patients in intensive care units. Despite intensive therapy, a high proportion of these patients contract systemic mycoses. CanTrain attempts to establish approaches leading to novel anti-fungal compounds by combining the expertise of 11 partners in cellular sensor systems and signalling pathways controlling morphogenesis and virulence, model systems for host-pathogen interaction, assay development and drug screening technologies. This network will not only give insight into mechanisms of host-pathogen interactions in general, but also bridge the gap between the identification of potential target proteins from the pathogen required for colonisation and infection of the host. CanTrain will enable the identification of antifungal compounds by establishing intelligent cell-based screening assays considering the host environment. These cell based screening assays will employ reporter genes activated by signalling pathways required for pathogenesis. The experimental approaches of CanTrain comprise state-of-the-art technologies, including global transcriptome and proteome analysis of pathogens (Candida albicans and Candida dubliniensis) during the infection process using in vitro host-pathogen generated tissue systems. The knowledge originating from this network will provide highly qualified individuals for the challenging tasks in life science aimed at antifungal drug discovery.


[+] Read More