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Background:
Salmonellosis
is one of the major causes of human disease related to food consumption.
Poultry products are the main source of human toxi-infections,
mostly because of an asymptomatic carrier-state (i.e. persistence
of Salmonella in apparently healthy fowls several weeks after
contamination or experimental inoculation). Most often the serotype
responsible for human food poisoning is Salmonella enteritidis.
The caecal and ovarian Salmonella carrier-states will be considered.
The former results in horizontal transmission of the bacteria
and leads to human disease through contamination of the egg shell
at the oviposition and of the carcass during evisceration. The
latter results in vertical transmission of Salmonella and in yolk
contamination. It has been shown that resistance to Salmonella
infection and carrier-state was partly genetically controlled.
Major genes of resistance to infection have been identified whereas
genes controlling resistance to carrier-state are still unknown.
Objectives:
Our
objectives are therefore:
1)
to discover which genes control the caecal carrier-state after
inoculation with Salmonella enteritidis;
2)
to find out to what extent these genes also control resistance
to the ovarian carrier-state after inoculation with Salmonella
enteritidis;
3)
to study the mode of action of these genes to help in their
identification and their use for selection.
Description:
In
the longer term (beyond the limits of this project) these results
will be transferred (by comparative genetics) to other animal
species also susceptible to Salmonella, but which lend themselves
less favourably to genetic studies. We shall first measure in
a commercial poultry line the effects of the resistance genes
already identified in mice (TASK 1) or mapped in fowls when studying
resistance to mortality (TASK 2). As the genes controlling resistance
to carrier-state and resistance to mortality partly differ, carrier
state QTL mapping will be achieved in TASK 3, to discover other
genome regions (or Quantitative Trait loci) involved. In TASK
4 we shall identify the differences in host response to Salmonella
enteritidis between genetically resistant and susceptible animals:
this will lead us to understand the mode of action of these genes
and will contribute to their identification. In TASK 5 we shall
determine to what extent genetic resistance will be efficient
against other Salmonella strains or serotypes. At the end of this
project we will know how to perform marker-assisted selection
for increased resistance to carrier-state, and what advantages
to expect from such a genetic improvement.
Current
situation/results :
The
work that had been planned has been achieved and analyses are
in progress.
Website:
http://www.tours.inra.fr/tours/sra/contratEC.htm
Coordinator
Catherine
BEAUMONT
Institut
National de la Recherche Agronomique-EPST
Rue
de l'Université 147
F-75338
Paris
Tel.:
+33 2 47 42 78 29
Fax:
+33 2 47 42 77 78
E-mail:
beaumont@chambord.tours.inra.fr
Partners
- Danielle MALO
The Montreal General Hospital Research Institute
(Mc Gill University Affiliated Hospital)
Avenue Cedar 1650
Room L11-144
Montreal QC
CDN-H3G 1A4
Tel.: +51 49 37 60 11
Fax: +51 49 34 82 61
E-mail: mc76@musica.mcgill.ca
- Paul Andrew BARROW
Institute for Animal Health
Compton
UK-RG2O 7NN Newbury
Tel.: +44 1635 57 84 11
Fax: +44 1635 57 72 37
E-mail: paul.barrow@bbsrc.ac.uk
- Jacob Anthony WAGENAAR
Institute for Animal Science and Health
Edelhertweg 15
PO Box 65
NL-8200 AB Lelystad
Tel.: +31 320 23 81 57
Fax: +31 320 23 81 53
E-mail: j.a.wagenaar@id.dlo.nl
- Philippe BARET
Université Catholique de Louvain
Place de l'Université 1
B-1348 Louvain-La-Neuve
Tel.: +32 10 47 37 23
Fax: +32 10 47 37 28
E-mail: baret@gena.ucl.ac.be
- Pierre COLIN
Agence Française de Sécurité Sanitaire
des Aliments
Avenue du Général De Gaulle 23
F-94701 Maisons Alfort
Tel.: +33 1 49 77 27 93
Fax: +33 1 49 77 90 05
E-mail: p.colin@dg.afssa.fr
- Michel Protais
Hubbard ISA SA
Avenue Maréchal de Saxe 119
F-69427 Lyon
Tel.: +33 2 96 79 63 80
Fax: +33 2 96 74 04 71
E-mail: Michel.Protais@Merial.com
- Martin WOODWARD
Veterinary Laboratories Agency
New Ham
UK-KT15 3NB Addlestone
Tel: +44 1932 35 70 00
Fax: +44 1932 34 70 46
E-mail: aq84@gtnet.gov.uk
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