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Non-specific serological reactions in bovine brucellosis. Development of diagnostic tools for differentiation between brucellosis and infection due to yersinia enerocolitica o:9

Last updating 1999

Contract nr: FAIR-CT95-0134
Project nr: 134
Project type: SC
Starting date: 01/04/1996
Duration: 36 months
Total cost: 2,264,000 EUR
EC Contribution: 1,910,000 EUR
Scientific Officer: Isabel MINGUEZ-TUDELA
Research topic: Animal health
Acronym: NON SPECIFIC SEROLOGICAL REACT

Background:
Bovine brucellosis (BB) has been eradicated in most northern EU countries but herds must be monitored continously for the potential presence of the disease. An unacceptable prevalence of the infection persists in certain areas of EU member states. A high level of non-specific reactions to serological tests has recently emerged in several countries. Another bacteria, Yersinia enterocolitica O:9 (YO9), seems to be responsible for these cross-reactions. Unnecessary restrictions, waste of resources, genetic losses, failure to control the disease, as well as trade restrictions are some of the consequences of this situation.

Objectives:
This proposal aims at developing diagnostic tests able to differentiate infection by Brucella abortus (BA) from infection by YO9. Blood tests based either on cellular or humoral immunity detecting only the anti-BA response as well as tests to detect specifically infections due to YO9 will be developed.

Description:
The project will consist of 7 main tasks: The development of a bovine gamma-interferon enzyme immunoassay for Brucella; of ELISA tests specific to Brucella; of an indirect ELISA using antigens specific to Yersinia; of immuno-affinity enrichment to improve direct bacteriological isolation of Yersinia; of a polymerase chain reaction-gene probe detection system specific to pathogenic strains of Yersinia; the experimental infection of cattle; and field trials.


Coordinator
Bruno GARIN-BASIUJI
CNEVA - Centre National d'Etudes Vétérinaires et Alimentaires
Rue Pierre Curie 22 - B.P. 67
F-94703 Maisons-Alfort
Tel.: +33 1 49 77 13 23
Fax: +33 1 43 68 97 62
E-mail: b.garin@alfort.afssa.fr


Partners

  • Jean-Jacques BENET
    Ecole Nationale Vétérinaire d'Alfort
    Av. du Général De Gaulle, 7
    F-94704 Maisons-Alfort
    Tel.: +33 1 43 96 70 08
    Fax: +33 1 43 96 71 31

  • Jacques GODFROID
    Institut National de Rrecherches Vétérinaires
    Groeselenberg, 99
    B-1180 Bruxelles
    Tel.: +32 2 375 44 55
    Fax: +32 2 375 09 79

  • Alastair Paul MACMILLAN
    Central Veterinary Laboratory
    Woodham Lane
    UK-KT15 3NB New haw - Addlestone
    Tel.: +44 1932 35 72 16
    Fax: +44 1932 35 75 95
    E-mail: cvl@dircon.co.uk

  • Gerard DUBRAY
    I.N.R.A. - Centre de Recherche de Tours
    F-37380 Nouzilly
    Tel.: +33 2 47 42 78 88
    Fax: +33 2 47 42 77 79
    E-mail: gerard.dubray@tours.inra.fr
  • Francis MILWARD
    Rhône Merieux SA
    Rue Marcel Merieux, 254 - B.P. 7009
    F-69342 Lyon
    Tel.: +33 4 72 72 34 32
    Fax: +33 4 72 72 34 98

  • Jean-Jacques LE TESSON
    Facultés Universitaires Notre-Dame de la Paix
    Rue de Bruxelles, 61
    B-5000 Namur
    Tel.: +32 81 72 44 02
    Fax: +32 81 72 44 20

  • Antonius J.M. OLYHOEK
    I.D. - D.L.O. - Institute for Animal Science and Health Research
    Edelhertweg, 15
    NL-8219 PH Lelystad
    Tel.: +31 320 23 84 01
    Fax: +31 320 23 80 50
    E-mail: a.j.m.olyhoek@id.agro.nl

  • George ZERVAS
    Agricultural University of Athens
    Iera Odos street 75
    GR-11855 Athens-Votanikos
    Tel.: +30 1 529 44 15
    Fax: +30 1 529 44 13

 
 
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