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BIODIOL
Process development for microbial production of the bulk chemical 1.3-Propanediol from glycerol water as a waste effluent

The aim of Biodiol is to develop an integrated biotechnical process for the production of the bulk-chemical 1.3-propanediol (PD) from glycerol waters by using the biological waste directly from biodiesel plants. The use of wastes from food industries as a co-substrate will be investigated to increase PD yield. To enable the usage of these cheap impure substrates, necessary desalting will be done by electrodialysis. A new, highly product-tolerant strain will be used in immobilised form for enhanced productivity and process stability, and to gain high PD concentrations, a (fed-) batch system will be set up. For process control, an online-sensor will be developed to monitor the concentration of the substrates. To prevent bacteria inhibition and to facilitate PD recovery, organic acids as by-products will be removed by electrodialysis.

Objectives
The objectives of the project are:
1) to have the micro-organism IK123 producing propanediol on a basis of glycerol water from biodiesel production with a molar yield of >90% when using co-substrates
2) to reach a final PD concentration of >110g/L
3) to provide an electrodialysis unit suitable for the desalination of crude (co-) substrates
4) to have a sensitive biosensor for reliable online-analysis available
5) to develop an optimal fermentation procedure including the feeding of substrates and using cheap media
6) to have electrodialysis continuously remove inhibiting by-products like organic acids
7) to increase the activity of the bioprocess by using a suitable immobilisation procedure
8) to have a complete economical validation of the integrated process.

Progress to Date
The first two deliverables have been achieved and work is being carried out according to the project plan. Tests with the first raw glycerols obtained from biodiesel industry and different co-substrates are in progress. A FIA biosensor is being developed for the fermentation process, copying fermentation requirements.

Several substitutes for the expensive yeast extract were found. Corn steep powder, cheaper technical yeast extract and vitamin solutions proved to be suitable. Yeast extract was significantly reduced without much loss in product concentration and yield. After an economical rating, a decision will be made on these strategies.

Classified in SOIL, NON-FOOD PRODUCTS

Scientist responsible for the project

Professor KLAUS-DIETER VORLOP
Bundesallee 50
38116 Braunschweig
Germany - DE

Phone: +49 5315964102
Fax: +49 5315964199
E-mail: klaus.vorlop@fal.de

References

Project ID QLRT-2001-01343
Organisation Federal Agricultural Research Centre
Area 5.2
Start date 02 January 2003
Duration (months) 36
Total cost 927 607 €
Total EC contribution   715 008 €
Status Ongoing
Web address of the project   http://www.biodiol.org

The partners

  • INSTITUTE OF CHEMISTRY, SLOVAK ACADEMY OF SCIENCES, Slovakia - SK
    chemhirs@savba.sk
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