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Detal
Fluorescent rapid and ultra-sensitive detection test for the tracking of toxic algal species in the marine environment

Harm fulalgal blooms have become an increasing problem in coastal waters all over the world. The objective of this proposal is to develop a new automated method for the detection of different harmful algal species. This methodology is a fluorescent-based technology for the rapid and sensitive detection of harmful algae, and has the potential to reduce the time of a test to less than three hours. It is based on the combination of the ChemScan technology developed by a biotechnology company and the expertise of five European research institutes. The introduction of this technology in monitoring programmes will allow a considerable increase in the number of sites and the frequency of the samplings. The HAB data collected from these programmes will be invaluable for evaluating the impact of the environmental changes on the development of toxic algae.

Objectives
To collect and cultivate harmful algae from three selected genera.
To test and design oligonucleotide probes for the detectionof harmful algal species in the marine environment.
To prepare monoclonal antibodies specific to surface antigens of toxic algae, for detection of harmful algal species in the marine environment.

Results
New technology will be used to complete the project objectives, including laser probes in a solid phase cytometer, which should speed up detection and identification work.

Classified in FISHERIES AND AQUACULTURE, GENOMICS

Scientist responsible for the project

Mr HERVÉ MOREAU
BP 44
66651 Banyuls-sur-mer CEDEX Cedex
France - FR

Phone: +33 4 68 88 73 73
Fax: +33 4 68 88 73 95
E-mail: h.moreau@obs-banyuls.fr

References

Project ID QLRT-1999-30778
Organisation Université Paris VI - Pierre et Marie Curie
Area 5.1.2
Start date 01 October 2000
Duration (months) 36
Total cost 1 645 183 €
Total EC contribution   1 030 000 €
Status Unknown

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