Antimicrobial resistance (AMR) is a rising threat to human and animal health and requires urgent and concerted action to limit its development and to maintain an arsenal of effective antimicrobials. This report presents an overview of the measures taken in the EU and beyond to promote the prudent use of antimicrobials in animals, as advocated in the relevant Union guidelines. These measures also include biosecurity and vaccination for disease prevention, control and eradication. Encouragingly, there are signs that these holistic approaches have led to decreasing levels of AMR. The report highlights potential examples of good practice which may be useful to other countries in shaping national policies on prudent use, as well as some challenges. The report also describes the Commission's current and future plans in this area, in particular new EU legislation on veterinary medicinal products and medicated feed.