Researcher - Differentiation of mouse ES/EpiS and hman iPS cells into cardiac, endothelial and smooth muscles.Offer by Large Company/SME
Researcher on Stem cell differentiation is available within the "Marie Curie Actions - Initial Training Networks" program of EU.
Objectives: Identification of environmental programming effects in prenatal stages on gene/metabolic pathways, stress resistance and differentiation
Tasks and methodology: -Comparing mouse ES/EpiS cell lines generated from mouse embryos exposed to variable in vitro culture conditions (nutrient changes, cell lines provided by USH) and their differentiation capability into cardiac, endothelial and smooth muscles in vitro (using established methods at BIO, fluorescent microscopy, flow cytometry, multiplate reader, real time RT PCR, in vivo fluorescent imaging).
-Generation of human iPS cell lines and their differentiation as above.
-Exposure of the above cell lines and differentiated cells to H2O2 and study of their survival and cell death (fluorescent microscopy and flow cytometry).
Results: i) Results on the effect of nutrient changes on the differentiation potential and stress resistance of mouse ES/EpiS cells.
ii) Effect of genetic reprogramming on the differentiation potential of hiPS cells and on their resistance to oxidative stress.
Experience in Human / Mouse cell culture and fluorescent microscopy is a MUST.
Experience in molecular biology (cloning, PCR), flow cytometry and stem cell culture is highly preferred.
Proficiency in Englisg is required.
Nr of positions available : 1
Biological sciences - Biology
Early stage researcher or 0-4 yrs (Post graduate)
First Stage Researcher (R1)
According to the Marie Curie Rates
To better plan and organise their stay in a foreign European country, researchers and their families can also benefit of the free and personalised assistance offered by the EURAXESS Services Centres, a network of more than 200 centres located in 40 different European countries.
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