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ESR project 2 stems from collaboration between WU-PBR and NIAB-TAG to search for natural sources of gluten with reduced toxicity for celiac disease patients. Variation is sought in protein epitopes of the gliadin genes located on the D genome (chromosome 6D) which are known to be particularly toxic for celiac patients. Two reverse genetic strategies are proposed for the current project. Cutting-edge site directed mutagenesis with Transcription Activator-Like Effector Nuclease (TALEN) constructs ...
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