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The main objective of the project is to evaluate the associations between standard (PM10, NOx etc.) and investigated (PAHs and nitroPAHs) parameters of air pollution and early effect biomarkers, and to propose a model for estimating the global risk of early biological effect due to air pollutants and other factors in children. The model will provide information that is valuable for guiding policy-making and planning individual and community interventions to protect children from possible health effects of air pollutants. This objective will be achieved using two biomarkers of early biological effects, DNA damage highlighted with the comet assay and the presence of micronuclei, in oral mucosa cells of 6-8-year-old children, and the following exposure variables, as possible risk factors: a) some airborne pollutants; b) air mutagenicity and toxicity, measured by in-vitro tests, and c) demographic and socio-economic variables, exposures to other pollutants and life-style
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