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One of the main constraints the SIT technology applied to mosquitoes currently suffers is the lack of an efficient sexing method to be applied in mass rearing facility. This proposal is therefore aimed at exploring the possibility to develop a new sexing non-GM method. The main activities planned are:
- rearing Ae. albopictus colonies in highly standardized condition and separation of male from female pupae by mechanical sieving (24 hrs from onset of pupation);
- acquiring data on the effect of specific components of the larval diet and larval density on the size of the pupae produced in order to explore the possibility to increase the size difference between male and female pupae and possibly enhancing the protandry;
- analyzing the protandry of Ae. albopictus and exploring the possibility to enhance time development differences between males and females (e.g. through the selection of strains obtained by crossing males that pupate earlier with the females that pupate later).
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Agricultural sciences - Other
Environmental science - Other
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