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The objective of this research is to identify and characterize the mRNAs of the genes
encoding the synthase analyzing a collection of 150 genotypes of durum wheat.
The profiles of transcription of each gene, relative to the various wheat genotypes, will be analyzed by real time quantitative PCR assays (rtq-PCR).The data of Real Time PCR allow to correlate the efficiency of expression of the genes with the specific character. These data will be integrated with a comparison study of the 5'- and 3'-UTR regions of the mRNA expressed by different alleles in order to identify potential regulatory elements that influence the efficiency of translation, stability and subcellular localization. The mapping of the 5'-regions and 3'-UTR will be realized by means of circular RT-PCR assays (cRT-PCR).
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