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Arbuscular mycorrhizal fungi (AMF) host endobacteria inside their cytoplasm. On the basis of the genome sequencing of Ca. Glomeribacter (Ghignone et al., 2012) the proposal is aimed to investigate some functional basis of the symbiosis between the endobacterium and its fungal host, using a combination of large-scale and targeted approaches. The two-years proposed research will focus on the following points: 1. High throughput transcriptomics of the G. margarita fungus using Next Generation Sequencing techniques (RNA deep sequencing) and Microarray design, comparing the profiles from the wt and the cured line . 2. Molecular analysis of bacterial factors that may play a functional role in the symbiosis with the fungal host by i) characterizing hypothetical "effector-coding genes" in the Ca. Glomeribacter genome with Yeast two-hybrid experiments and ii) genes coding for the NRPS/PKS machinery responsible for the synthesis of bioactive compounds with a potential antibiotic activity.
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The call and how to apply are available at the following address: https://www.serviziweb.unito.it/albo_ateneo/
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