The publication of "The Principles of Humane Experimental Technique" by W.M.S. Russell and R.L. Burch in 1959 marks the birth of the principle of the “Three Rs”.
The authors proposed the principles of Replacement, Reduction and Refinement (the “Three Rs”) as the key strategies of a systematic framework aimed at achieving the goal of humane experimental techniques. Russell and Burch saw replacement as the ultimate goal for laboratory animal based research, education and testing, with the other two, reduction and refinement, being more readily achievable in the short term.
Replacement may be achieved through a number of tools or their combinations including
Today, the term refinement signifies the modification of any procedures or husbandry and care practices from the time the experimental animal is born until its death, so as to minimise the pain, suffering and distress experienced by the animal and enhance its well-being.
When an animal experiences pain, suffering or distress, there are often accompanying physiological changes which may increase the variability of scientific results. Refinement therefore is also likely to improve data quality and contribute to Reduction.
The principle of the “Three Rs” has been present in the EU legislation in spirit, from as early as 1986, when the first EU legislation for the protection of animals used for experimental and other scientific purposes was adopted.
However, Directive 2010/63/EU on the protection of animals used for scientific purposes, for the first time in EU legislation spells out the principle of the “Three Rs” and makes it a firm legal requirement. The principles of Replacement, Reduction and Refinement must be considered systematically at all times when animals are used for scientific purposes in the EU. Under the Directive, the term "scientific purposes" covers all uses of animals for the purposes of basic, translation and applied research, regulatory testing and production as well as for the purposes of education and training.
Furthermore, the Directive ensures that its application goes beyond that of the original, narrower interpretation of the “Three Rs” only in the context of choice of methods. The Directive enlarges the Refinement also to cover all animal breeding and care – that is, to ensure refinement during housing, breeding and care even if the animal is not undergoing a scientific procedure.
The term ‘alternatives’ in this context includes all assays, tests, methods, techniques, tools, strategies and approaches etc. that contribute to the practical implementation of the “Three Rs”. That is
Refinement can also be achieved by moving from species that are considered more sentient to those less sentient. Examples: substituting a non-human primate by the use of a fish or substituting the use of fish with daphnia. These are both considered methods of refinement as they are likely to reduce the pain, suffering and distress experienced by the animal, however, still requiring the use of live animals.
Alternative methods provide opportunities to advance the "Three Rs", but equally aim at developing better and more predictive scientific tools to protect human and animal health and the environment.